Arachidonoyl p-Nitroaniline

Arachidonoyl p-nitroaniline (ApNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity. FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its nominal endogenous substrate anandamide (AEA, Cay-90050). It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. (See also Decanoyl p-Nitroaniline - Catalog No. 90349). Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (epsilon = 13,500 at 382 nm). This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer.Formal Name: N-(4-nitrophenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide. CAS Number: 119520-58-0. Synonyms: ApNA. Molecular Formula: C26H36N2O3. Formula Weight: 424.6. Purity: >98%. Formulation: (Request formulation change), A solution in methyl acetate. Solubility: DMF: 50 mg/ml, DMSO: 50 mg/ml, DMSO:PBS (pH 7.2) (1:4): 1 mg/ml, Ethanol: 50 mg/ml. lambdamax: 204, 316 nm. SMILES: CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(=O)Nc1ccc(cc1)[N](=O)O. InChi Code: InChI=1S/C26H36N2O3/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-26(29)27-24-20-22-25(23-21-24)28(30)31/h6-7,9-10,12-13,15-16,20-23H,2-5,8,11,14,17-19H2,1H3,(H,27,29)/b7-6-,10-9-,13-12-,16-15-. InChi Key: PBMVYDNBOIVHBO-DOFZRALJSA-N.