5alpha,6beta-Dihydroxycholestanol-d7

5alpha,6beta-Dihydroxycholestanol-d7 is intended for use as an internal standard for the quantification of 5alpha,6beta-dihydroxycholestanol (Cay-25538) by GC- or LC-MS. 5alpha,6beta-Dihydroxycholestanol is an oxysterol metabolite of cholesterol formed from conversion of cholesterol epoxides by 5,6-epoxysterol hydrolase. It inhibits NMDA-mediated calcium influx in HEK293 cells expressing NR1/NR2B NMDA receptors in a concentration-dependent manner. It also binds to voltage-gated sodium (Nav) channels and decreases action potentials in hippocampal neurons in vitro when used at a concentration of 10 µM. It increases survival of spinal cord motoneurons, cortical neurons, and cerebellar granule neurons in vitro when used at concentrations ranging from 5 to 15 µM. 5alpha,6beta-Dihydroxycholestanol is neuroprotective in a rat model of cerebral ischemia when administered at a dose of 12 mg/kg and increases latency to seizure onset and reduces severity of seizures induced by pentylenetetrazole (PTZ, Cay-18682) in rats. 5alpha,6beta-Dihydroxycholestanol has been used as a replacement for cholesterol in the study of cholesterol binding proteins.Formal Name: cholestane-25,26,26,26,27,27,27-d7-3beta,5alpha,6beta-triol. CAS Number: 127684-07-5. Synonyms: Cholestanetriol-d7, 5alpha,6beta-di-OHC-d7. Molecular Formula: C27H41D7O3. Formula Weight: 427.7. Purity: >99% deuterated forms (d1-d7). Formulation: (Request formulation change), A solid. Solubility: Methanol: Slightly Soluble. SMILES: C[C@H](CCCC(C([2H])([2H])[2H])([2H])C([2H])([2H])[2H])[C@@]1([H])CC[C@@]2([H])[C@]3([H])C[C@@H](O)[C@@]4(O)C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C. InChi Code: InChI=1S/C27H48O3/c1-17(2)7-6-8-18(3)21-9-10-22-20-15-24(29)27(30)16-19(28)11-14-26(27,5)23(20)12-13-25(21,22)4/h17-24,28-30H,6-16H2,1-5H3/t18-,19+,20+,21-,22+,23+,24-,25-,26-,27+/m1/s1/i1D3,2D3,17D. InChi Key: YMMFNKXZULYSOQ-UCHKBJBUSA-N.