13,14-dihydro-15-keto Prostaglandin E2-d4

13,14-dihydro-15-keto Prostaglandin E2-d4 (13,14-dihydro-15-keto PGE2-d4) is intended for use as an internal standard for the quantification of 13,14-dihydro-15-keto PGE2 (Cay-14650) by GC- or LC-MS. 13,14-dihydro-15-keto PGE2 is a metabolite of PGE2 (Cay-14010) and the primary PGE2 metabolite in plasma. It is formed from PGE2 via a 15-keto PGE2 intermediate by 15-oxo-PG Delta13 reductase. Unlike PGE2, 13,14-dihydro-15-keto PGE2 does not bind effectively to the PGE2 receptors EP2 and EP4 expressed in CHO cells (Kis = 12 and 57 µM, respectively) or induce adenylate cyclase activity in the same cells (EC50s = >18 and >38 µM, respectively). Levels of 13,14-dihydro-15-keto PGE2 are increased in the plasma of women in the third trimester of pregnancy and in women during and immediately after labor and delivery. Levels of 13,14-dihydro-15-keto PGE2 levels are decreased in tumor tissue compared to adjacent non-cancerous tissue isolated from patients with non-small cell lung cancer (NSCLC).Formal Name: 9,15-dioxo-11alpha-hydroxy-prost-5Z-en-1-oic-3,3,4,4-d4 acid. Synonyms: 13,14-dh-15-keto PGE2-d4, 13,14-dihydro-oxo-PGE2-d4, PGEM-d4. Molecular Formula: C20H28D4O5. Formula Weight: 356.5. Purity: >99% deuterated forms (d1-d4). Formulation: (Request formulation change), A solution in methyl acetate. Solubility: DMF: 50 mg/ml, DMSO: 50 mg/ml, Ethanol: 50 mg/ml, PBS (pH 7.2): 1 mg/ml. SMILES: O[C@H]1[C@H](CCC(CCCCC)=O)[C@@H](C/C=C\C([2H])([2H])C([2H])([2H])CC(O)=O)C(C1)=O. InChi Code: InChI=1S/C20H32O5/c1-2-3-6-9-15(21)12-13-17-16(18(22)14-19(17)23)10-7-4-5-8-11-20(24)25/h4,7,16-17,19,23H,2-3,5-6,8-14H2,1H3,(H,24,25)/b7-4-/t16-,17-,19-/m1/s1/i5D2,8D2. InChi Key: CUJMXIQZWPZMNQ-BQWYWOLISA-N.