Pentafluorobenzenesulfonyl fluorescein

Reactive oxygen species (ROS) play important roles in the initiation and progression of many disease processes. Most fluorescent probes for ROS detection, such as 2,7-dichlorofluorescein (DCFH), function by an oxidative mechanism and are useful for total oxidant detection but are not selective for hydrogen peroxide specifically. Pentafluorobenzenesulfonyl fluorescein is a fluorescent probe that functions by a nonoxidative mechanism. It fluoresces upon perhydrolysis of the sulfonyl linkage, and is selective for hydrogen peroxide over hydroxyl radical, tert-butyl hydroperoxide, superoxide anion, singlet oxygen, and nitrates. Pentafluorobenzenesulfonyl fluorescein can be used as a probe for hydrogen peroxide in whole cell systems.Formal Name: pentafluorobenzenesulfonic acid, 6'-hydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl ester. CAS Number: 728912-45-6. Molecular Formula: C26H11F5O7S. Formula Weight: 562.4. Purity: >98%. Emission: 530 ±25 nm. Excitation: 485 ±20 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, DMSO:PBS (pH 7.2)(1:1): .25 mg/ml, Ethanol: 20 mg/ml. SMILES: FC1=C(F)C(F)=C(F)C(F)=C1S(OC2=CC=C(C3(OC(C4=CC=CC=C43)=O)C(C=CC(O)=C5)=C5O6)C6=C2)(=O)=O. InChi Code: InChI=1S/C26H11F5O7S/c27-19-20(28)22(30)24(23(31)21(19)29)39(34,35)38-12-6-8-16-18(10-12)36-17-9-11(32)5-7-15(17)26(16)14-4-2-1-3-13(14)25(33)37-26/h1-10,32H. InChi Key: UIBLLPHZQUTIBL-UHFFFAOYSA-N.