Coomassie Blue R-250

Coomassie blue R-250 is a dye that is commonly used in laboratories to stain or quantify proteins. It is a sensitive stain for protein detection in polyacrylamide gels, typically giving blue bands on a clear background with a sensitivity of 50-100 ng/band. It may be combined with other stains, such as silver stain, to distinguish different types of proteins. Coomassie blue R-250 displays metachromasia by appearing pink-red, rather than blue, when binding certain proteins, such as collagen and histone H1.Formal Name: N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-benzenemethanaminium, monosodium salt. CAS Number: 6104-59-2. Synonyms: C.I. 42660. Molecular Formula: C45H44N3O7S2 . Na. Formula Weight: 826.0. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 2 mg/ml, DMSO: 10 mg/ml, Ethanol: 0.1 mg/ml, PBS (pH 7.2): 1 mg/ml. SMILES: CC/[N+](CC1=CC(S([O-])(=O)=O)=CC=C1)=C(C=C/2)\C=CC2=C(C3=CC=C(N(CC)CC4=CC=CC(S([O-])(=O)=O)=C4)C=C3)\C(C=C5)=CC=C5NC6=CC=C(OCC)C=C6.[Na+]. InChi Code: InChI=1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54,/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53. InChi Key: NKLPQNGYXWVELD-UHFFFAOYSA-M. Origin: Synthetic.