Biological Buffers

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing protons. The pH value is kept constant even after addition of acids of bases. This is of special importance with regards to biochemical reactions: The charge of nucleic acids and proteins is strongly correlated to the local pH. Biological solutions like blood or saliva are therefore buffered, mostly by phosphate- or carbonate buffer systems. In molecular biology and biochemistry buffering is achieved by addition of so called “good buffers” or PBS.

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing... read more »
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Biological Buffers

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing protons. The pH value is kept constant even after addition of acids of bases. This is of special importance with regards to biochemical reactions: The charge of nucleic acids and proteins is strongly correlated to the local pH. Biological solutions like blood or saliva are therefore buffered, mostly by phosphate- or carbonate buffer systems. In molecular biology and biochemistry buffering is achieved by addition of so called “good buffers” or PBS.

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Bis-Tris-Propane, pure
Bis-Tris-Propane, pure

Item number: 50224.100

Useful pH range: 6.3-9.5
CAS 64431-96-5
MW: 282.40
From 199.00€ *
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Tris Base Ultrapure (Tris(hydroxymethyl) aminomethane, 2-Amino-2-(hydroxymethyl)-1, 3-propanediol, T
Tris Base Ultrapure (Tris(hydroxymethyl) aminomethane,...

Item number: T8600.1

Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity. Synonyms: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, 2-Amino-2-(hydroxymethyl)propane-1,3-diol, 2-Amino-2-methylol-1,3-propanediol, Addex-Tham, Aminotri(hydroxymethyl)methane,...
Keywords: Trometamol, Tromethamin, Tris(hydroxymethyl)-aminomethan, 2-Amino-2-(hydroxymethyl)-propan-1,3-diol
CAS 77-86-1
MW: 121.14 D
From 56.00€ *
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TES, ultra pure
TES, ultra pure

Item number: 22080.500

Useful pH range: 7.0-8.0
CAS 7365-44-8
MW: 229.18
From 117.00€ *
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Tris Base USP (Tris(hydroxymethyl) aminomethane,_2-Amino-2-(hydro xymethyl)-1, 3-propanediol,_Tromet
Tris Base USP (Tris(hydroxymethyl)...

Item number: T8601.1

Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity. Our USP Grade is very similar in specifications to ultrapure Tris (T8600). Purity: >99.0%, Appearance: White, crystalline powder, Solubility (1M): Colorless, clear,...
Keywords: Trometamol, Tromethamin, Tris(hydroxymethyl)-aminomethan, 2-Amino-2-(hydroxymethyl)-propan-1,3-diol
CAS 77-86-1
MW: 121.14 D
From 49.00€ *
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MES Free Acid (2-(N-Morpholino) ethanesulfonic acid)
MES Free Acid (2-(N-Morpholino) ethanesulfonic acid)

Item number: M3000.250

MES Free Acid is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 4432-31-9
MW: 195.20 D
From 242.00€ *
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HEPES Sodium Salt (N-[2-Hydroxyethyl] piperazine-N'- [2-ethanesulfonic acid] sodium salt)
HEPES Sodium Salt (N-[2-Hydroxyethyl] piperazine-N'-...

Item number: H2030.500

HEPES Sodium is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 75277-39-3
MW: 260.30 D
From 90.00€ *
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MES, Sodium Salt (2-(N-Morpholino) ethanesulfonic acid sodium salt)
MES, Sodium Salt (2-(N-Morpholino) ethanesulfonic acid...

Item number: M3005.100

MES Sodium Salt is a biological buffer (zwitterionic) designed by Good et al., and typically referred to as Good's buffer. It is useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between...
CAS 71119-23-8
MW: 217.20 D
From 130.00€ *
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Tris Acetate (Tris(hydroxymethyl) aminomethane acetate, 2-Amino-2-(hydroxymethyl)-1, 3-propanediol a
Tris Acetate (Tris(hydroxymethyl) aminomethane acetate,...

Item number: T8600-05.100

Tris acetate is used for the production of TAE buffer for molecular biology applications. Appearance: White, crystalline powder, Purity: >99%, Melting Point: 115-125°C, Solubility (1%, dH2O): Colorless, clear, complete , pH (1%, dH2O): 6.0-7.0, Absorbance: 250nm: <0.3, 260nm: <0.1, 280nm: <0.05, Chloride: <0.05%,...
CAS 6850-28-8
MW: 181.20 D
From 64.00€ *
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Tricine (N-[tris-hydroxymethyl]methyllglycine)
Tricine (N-[tris-hydroxymethyl]methyllglycine)

Item number: T8400.250

Tricine is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 5704-04-1
MW: 179.20 D
From 174.00€ *
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Phosphate Buffer, APHA, pH 7.2 (Butterfield's Buffered Phosphate Diluent) (Powder)
Phosphate Buffer, APHA, pH 7.2 (Butterfield's Buffered...

Item number: P4071-50.100

Intended Use:, Phosphate Buffer, pH 7.2 is used for the preparation of microbiological dilution blanks. Product Summary: The formula for phosphate buffer is specified by the American Public Health Association (APHA) for use in diluting test samples. Phosphate Buffer, pH 7.2 is specified for use in diluting water,...
From 116.00€ *
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PIPES Free Acid (Piperazine-N,N'-bis(2-ethane sulfonic acid))
PIPES Free Acid (Piperazine-N,N'-bis(2-ethane sulfonic...

Item number: P4200.250

PIPES is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240-700nm, chemically...
CAS 5625-37-6
MW: 302.40 D
From 98.00€ *
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TAPSO Free Acid (3-[N-tris-(hydroxymethyl) methylamino]-2- hydroxypropanesulfonic acid)
TAPSO Free Acid (3-[N-tris-(hydroxymethyl)...

Item number: T1010.1

TAPSO Free Acid is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 68399-81-5
MW: 259.30 D
From 104.00€ *
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