SMI-481

Phosphatidylinositol (PtdIns) transfer proteins (PITPs) mediate the exchange of PtdIns and phosphatidylcholine between membranes, a step required for a wide array of cellular processes including cell division. SMI-481 is an inhibitor of the yeast PITP Sec14, blocking both Sec14-mediated transfer of [3H]-PtdIns in vitro and growth (IC50s = 0.21 and 2.87 µM, respectively). Suppression of Sec14 expression leads to proportional reduction in SMI-481 inhibition, suggesting that SMI-481 acts specifically through Sec14. Moreover, SMI-481 does not affect [3H]-PtdIns transfer mediated by other yeast PITPs.Formal Name: (4-chloro-3-nitrophenyl)[4-(2-fluorophenyl)-1-piperazinyl]-methanone. CAS Number: 432020-20-7. Molecular Formula: C17H15ClFN3O3. Formula Weight: 363.8. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 50 mg/ml, DMF:PBS (pH 7.2) (1:4): 0.2 mg/ml, DMSO: 30 mg/ml, Ethanol: 0.3 mg/ml. lambdamax: 240 nm. SMILES: FC1=C(N2CCN(C(C3=CC([N+]([O-])=O)=C(Cl)C=C3)=O)CC2)C=CC=C1. InChi Code: InChI=1S/C17H15ClFN3O3/c18-13-6-5-12(11-16(13)22(24)25)17(23)21-9-7-20(8-10-21)15-4-2-1-3-14(15)19/h1-6,11H,7-10H2. InChi Key: LSPJXCGEFJDMHA-UHFFFAOYSA-N.