ReadiUse(TM) TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

Ex: 650 nm. Horseradish peroxidase (HRP) and HRP conjugates facilitate the ABTS oxidation in the presence of hydrogen peroxide, turning ABTS into its blue-green oxidized product. ReadiUse(TM) TMB Substrate Solution is a premixed solution of TMB substrate with hydrogen peroxide. It produces a blue product upon interaction with HRP or HRP conjugates without the addition of hydrogen peroxide. The soluble blue product can be quantitated at 650 nm. Use of a stop solution enhances sensitivity 2-4 fold and the resulting yellow solution can be read at 450 nm. ReadiUse(TM) TMB Substrate Solution provides an convenient and ultrasensitive quantitative substrate system.