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Monobromobimane is a thiol-reactive fluorogenic probe. It is cell-permeable, reacts rapidly at physiological pH with available thiol groups, and generates a stable fluorescent signal. Monobromobimane can be used to evaluate or quantify a variety of compounds containing reactive sulfur or thiol groups, including H2S, glutathione, proteins, and nucleotides. The absorption and emission maxima for monobromobimane are 398 and 490 nm, respectively.Formal Name: 3-(bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione. CAS Number: 71418-44-5. Synonyms: MBBr, NSC 608544. Molecular Formula: C10H11BrN2O2. Formula Weight: 271.1. Purity: >98%. Emission: 490 nm. Excitation: 398 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 50 mg/m, DMSO: 50 mg/m, Ethanol: 1 mg/ml. lambdamax: 247, 383 nm. SMILES: O=C1N2N(C(CBr)=C(C)C2=O)C(C)=C1C. InChi Code: InChI=1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3. InChi Key: AHEWZZJEDQVLOP-UHFFFAOYSA-N.
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