KH7

Soluble adenylyl cyclase mediates bicarbonate-stimulated production of the ubiquitous second messenger adenosine 3',5'-cyclic mononucleotide (cAMP). It is abundantly expressed in sperm, in fluid transporting tissues such as kidney cortex and medulla, and in other bicarbonate-responsive tissues and cells. KH7 is a selective inhibitor of soluble adenylyl cyclase (sAC) that has little effect on transmembrane adenylyl cyclases. It displays an IC50 value between 3-10 µM toward sAC. At 50 µM, KH7 decreases basal cAMP accumulation in sperm. KH7 has been used in diverse research applications including cAMP-mediated signaling events required for mammalian egg fertilization, sAC regulation of Na+ transport in the kidney, and mitochondrial-dependent apoptosis in response to various stress stimuli. KH7 is not active against sAC in the presence of detergents. At concentrations above 50 µM, KH7 exhibits non-specific membrane disruption effects when used on cells.Formal Name: 2-(1H-benzimidazol-2-ylthio)-2-[(5-bromo-2-hydroxyphenyl)methylene]hydrazide, propanoic acid. CAS Number: 330676-02-3. Molecular Formula: C17H15BrN4O2S. Formula Weight: 419.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 25 mg/ml, Ethanol: 0.5 mg/ml. lambdamax: 284, 337 nm. SMILES: OC1=CC=C(Br)C=C1/C=N/NC(C(C)([H])SC2=NC3=C(C=CC=C3)N2)=O. InChi Code: InChI=1S/C17H15BrN4O2S/c1-10(25-17-20-13-4-2-3-5-14(13)21-17)16(24)22-19-9-11-8-12(18)6-7-15(11)23/h2-10,23H,1H3,(H,20,21)(H,22,24)/b19-9+. InChi Key: WILMXUAKQKGGCC-DJKKODMXSA-N.