FunXite(TM)-1

FunXite(TM)-1 is chemically same molecule to FUN(R) 1 (FUN(R) 1 is the trademark of ThermoFisher). It is a unique two-color fluorescent viability probe for yeast and fungi. FunXite-1 diffuses into a variety of cells and initially stains the cytoplasm with a diffusely distributed green fluorescence. In some yeasts and fungi, the stain reacts with some cellular substances (e.g., glutathione) to generate distinct vacuolar structures that exhibit a striking red fluorescence, accompanied by a reduction in the green cytoplasmic fluorescence. Formation of the intravacuolar structures requires both plasma membrane integrity and metabolic capability. Dead yeast and fungus cells fluoresce bright yellow-green, with no discernable red structures.