Fluo-2 AM

Fluo-2 AM is a cell-permeable fluorescent calcium indicator. It has been used to measure the activity of the neuropeptide Y4 receptor, as well as to detect intracellular calcium in tetrandine-stimulated primary rabbit corpus cavernosum smooth muscle cells. It binds to calcium (Kd = 290 nM) and displays excitation/emission maxima of 490/515 nm, respectively. Fluo-2 AM is also available in a cell-impermeable form (Cay-35764).Formal Name: N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[4-[6-[(acetyloxy)methoxy]-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-glycine (acetyloxy)methyl ester. CAS Number: 1070771-36-6. Synonyms: Fluo-2 Acetoxymethyl ester, Fluo-2 HA, Fluo-2 High Affinity. Molecular Formula: C51H52N2O23. Formula Weight: 1061.0. Purity: >95%. Emission: 515 nm. Excitation: 490 nm. Formulation: (Request formulation change), A solid. Solubility: DMSO: Soluble. lambdamax: 254 nm. SMILES: O=C1C=CC2=C(C3=CC(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C3)C5=C(C=C(C=C5)OCOC(C)=O)OC2=C1. InChi Code: InChI=1S/C51H52N2O23/c1-30-7-13-41(52(21-47(60)72-26-67-32(3)55)22-48(61)73-27-68-33(4)56)45(17-30)64-15-16-65-46-18-36(8-14-42(46)53(23-49(62)74-28-69-34(5)57)24-50(63)75-29-70-35(6)58)51-39-11-9-37(59)19-43(39)76-44-20-38(10-12-40(44)51)71-25-66-31(2)54/h7-14,17-20H,15-16,21-29H2,1-6H3. InChi Key: ZGEIIQJBYATNMH-UHFFFAOYSA-N.