Dibenzylfluorescein

Faint to dark orange powder Soluble in DMSO (10mg/ml) or DMF (20mg/ml). Chemical. CAS: 97744-44-0. Formula: C34H24O5. MW: 512.55. Dibenzylfluorescein is a fluorogenic probe that acts as a substrate for specific cytochrome P450 (CYP) isoforms, including CYP3A4, CYP2C8, CYP2C9, CYP2C19 and aromatase (CYP19). It is dealkylated by these CYP isoforms to produce fluorescein benzyl ether, which is further hydrolyzed to fluorescein by the addition of base (typically 2 M NaOH). Dibenzylfluorescein is typically used near its apparent Km value of 0.87-1.9 µM. The fluorescence of fluorescein is evaluated using excitation/emission wavelengths of 485/538nm. Dibenzylfluorescein is used to detect changes in CYP catalytic activity caused by drugs or disease.