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DEPMPO is a nitrone that is used to spin trap reactive O-, N-, S-, and C-centered radicals and allow their characterization when used in association with electron spin resonance. It is noted for the stability of adducts formed. DEPMPO can be used in vitro or in vivo, as it crosses lipid bilayer membranes and is a good trapping agent in biological systems. DEPMPO-biotin is a biotinylated form of DEPMPO which retains the outstanding persistency of its adducts. The biotin moiety offers an effective means for monitoring biodistribution in cells, tissues, and organs when used with an avidin-conjugated reporter. Importantly, DEPMPO-biotin binds free radicals, such as S-nitroso groups, on proteins, producing adducts that can be analyzed via the biotin tag. This direct labeling of S-nitrosothiols (SNO) thus serves as an effective alternative to the more cumbersome biotin-switch method for monitoring SNO formation.Formal Name: N-[3-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]propyl]-[(2S,3R)-2-(diethoxyphosphinyl)-3,4-dihydro-2-methyl-1-oxido-2H-pyrrol-3-yl]methyl ester-carbamic acid. CAS Number: 936224-52-1. Molecular Formula: C24H42N5O8PS. Formula Weight: 591.7. Purity: >95%. Formulation: (Request formulation change), A solution in ethanol. Solubility: Ethanol: 10 mg/ml, Water: 1 mg/ml. lambdamax: 202, 241 nm. SMILES: CCOP(=O)(OCC)C1(C)[C@H](COC(=O)NCCCNC(=O)CCCC[C@H]2SC[C@H]3NC(=O)N[C@@H]23)CC=[N+]1[O-]. InChi Code: InChI=1S/C24H42N5O8PS/c1-4-36-38(34,37-5-2)24(3)17(11-14-29(24)33)15-35-23(32)26-13-8-12-25-20(30)10-7-6-9-19-21-18(16-39-19)27-22(31)28-21/h14,17-19,21H,4-13,15-16H2,1-3H3,(H,25,30)(H,26,32)(H2,27,28,31)/t17-,18+,19-,21+,24?/m0/s1. InChi Key: UAQUUBGIOJZAFO-GLZOQUOBSA-N.
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the usability of this website, serve for direct advertising or simplify interaction with other websites and social networks, will only be used with your consent.
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