Cinobufotalin

Cinobufotalin is a steroid glycoside originally isolated from the Asiatic toad (B. gargarizans) and has diverse biological activities. It reduces SRC-3 protein levels in MCF-7 breast cancer cells when used at concentrations ranging from 10 to 100 nM. Cinobufotalin (1 µM) reduces Na+/K+ pump activity, viability, and cell attachment of C7-MDCK cells. It reduces expression of the epithelial-mesenchymal (EMT) markers E-cadherin, vimentin, ZEB1, and Slug in and migration and invasion of PC3 cells. Cinobufotalin (0.1-10 µM) is cytotoxic to A549, H460, and HTB-58 lung cancer cells. In vivo, cinobufotalin (1 and 5 mg/kg) reduces tumor volume and increases survival in an A549 mouse xenograft model.Formal Name: 16beta-(acetyloxy)-14,15beta-epoxy-3beta,5beta-dihydroxy-bufa-20,22-dienolide. CAS Number: 1108-68-5. Synonyms: NSC 90326. Molecular Formula: C26H34O7. Formula Weight: 458.6. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 10 mg/ml, DMF:PBS (pH 7.2) (1:1): 0.5 mg/ml, DMSO: 5 mg/ml, Ethanol: 5 mg/ml. lambdamax: 296 nm. SMILES: O[C@H]1CC[C@@]2(C)[C@@](CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@]3(O5)[C@H]5[C@H](OC(C)=O)[C@@H]4C6=COC(C=C6)=O)(O)C1. InChi Code: InChI=1S/C26H34O7/c1-14(27)32-21-20(15-4-5-19(29)31-13-15)24(3)10-7-17-18(26(24)22(21)33-26)8-11-25(30)12-16(28)6-9-23(17,25)2/h4-5,13,16-18,20-22,28,30H,6-12H2,1-3H3/t16-,17-,18+,20-,21+,22+,23+,24+,25-,26+/m0/s1. InChi Key: KBKUJJFDSHBPPA-ZNCGZLKOSA-N.