Calcein

Calcein is a xanthene that is commonly used for the fluorometric determination of calcium in solution. As this form of calcein is not membrane permeable, it can be used in assays that evaluate membrane integrity. Calcein can also be used as a fluorescent indicator for fluoride, iron, and mercury. Excitation and emission maxima for calcein are 494 and 517 nm, respectively.Formal Name: N,N'-[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl)bis(methylene)]bis[N-(carboxymethyl)-glycine. CAS Number: 1461-15-0. Synonyms: Fluorescein Complexone, NSC 298193. Molecular Formula: C30H26N2O13. Formula Weight: 622.5. Emission: 517 nm. Excitation: 494 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: 1 M NaOH: 50 mg/ml, PBS (pH 7.2): 0.2 mg/ml. lambdamax: 226, 278 nm. SMILES: OC1=CC2=C(C=C1CN(CC(O)=O)CC(O)=O)C3(C(C=CC=C4)=C4C(O3)=O)C5=CC(CN(CC(O)=O)CC(O)=O)=C(O)C=C5O2. InChi Code: InChI=1S/C30H26N2O13/c33-21-7-23-19(5-15(21)9-31(11-25(35)36)12-26(37)38)30(18-4-2-1-3-17(18)29(43)45-30)20-6-16(22(34)8-24(20)44-23)10-32(13-27(39)40)14-28(41)42/h1-8,33-34H,9-14H2,(H,35,36)(H,37,38)(H,39,40)(H,41,42). InChi Key: DEGAKNSWVGKMLS-UHFFFAOYSA-N.