4-Nitrophenyl-N-acetyl-beta-D-glucosaminide

4-Nitrophenyl-N-acetyl-beta-D-glucosaminide is a chromogenic substrate for N-acetyl-beta-glucosaminidase. Hydrolysis of 4-nitrophenyl-N-acetyl-beta-D-glucosaminide by N-acetyl-beta-glucosaminidase releases 4-nitrophenol, which can be quantified by colorimetric detection at 405 nm as a measure of N-acetyl-beta-glucosaminidase activity. It has been used to quantify the activity of N-acetyl-beta-D-glucosaminidase in human serum and urine in parenterally fed patients.Formal Name: 4-nitrophenyl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside. CAS Number: 3459-18-5. Synonyms: GlcNAc-PNP, p-Nitrophenyl-N-acetyl-beta-D-glucosaminide. Molecular Formula: C14H18N2O8. Formula Weight: 342.3. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 15 mg/ml, DMF:PBS (pH 7.2) (1:4): 0.2 mg/ml, DMSO: 3 mg/ml. lambdamax: 220, 296 nm. SMILES: O[C@H]([C@H]1NC(C)=O)[C@H](O)[C@@H](CO)O[C@H]1OC2=CC=C([N+]([O-])=O)C=C2. InChi Code: InChI=1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14-/m1/s1. InChi Key: OMRLTNCLYHKQCK-DHGKCCLASA-N.