4-Methylumbelliferyl-N-acetyl-alpha-D-Neuraminic Acid (sodium salt)

4-Methylumbelliferyl-N-acetyl-alpha-D-neuraminic acid is a fluorogenic substrate of neuraminidases. 4-Methylumbelliferyl-N-acetyl-alpha-D-neuraminic acid is cleaved by neuraminidases to release the fluorescence moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. 4-Methylumbelliferyl-N-acetyl-alpha-D-neuraminic acid has been used to quantify neuraminidase activity in mammalian cells and clinical isolates of influenza virus.Formal Name: 4-methyl-2-oxo-2H-1-benzopyran-7-yl 5-(acetylamino)-3,5-dideoxy-alpha-neuraminic acid, monosodium salt. CAS Number: 76204-02-9. Synonyms: 4-MUNANA, Neu5Ac-alpha-4MU, Sodium 2-(4-methylumbelliferyl)-N-acetylneuraminate. Molecular Formula: C21H24NO11 . Na. Formula Weight: 489.4. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 15 mg/ml, DMSO: 20 mg/ml, Ethanol: 0.25 mg/ml, PBS (pH 7.2): 10 mg/ml. lambdamax: 317 nm. SMILES: O=C1C=C(C)C2=CC=C(O[C@]3(C([O-])=O)C[C@H](O)[C@@H](NC(C)=O)[C@@]([C@H](O)[C@H](O)CO)([H])O3)C=C2O1.[Na+]. InChi Code: InChI=1S/C21H25NO11.Na/c1-9-5-16(27)31-15-6-11(3-4-12(9)15)32-21(20(29)30)7-13(25)17(22-10(2)24)19(33-21)18(28)14(26)8-23,/h3-6,13-14,17-19,23,25-26,28H,7-8H2,1-2H3,(H,22,24)(H,29,30),/q,+1/p-1/t13-,14+,17+,18+,19+,21+,/m0./s1. InChi Key: NNNXBDLJYKMDAI-NLSRWXBQSA-M.