ATF6 Luciferase Reporter Lentivirus (ATF6 Pathway)

The ATF6 (Activating Transcription Factor 6) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce most types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with the firefly luciferase gene driven by multiple copies of an ATF6 response element, located upstream of the minimal TATA promoter. The lentiviruses also transduce a puromycin selection gene (Figure 1). After transduction, the ATF6-mediated response to ER (endoplasmic reticulum) stress can be monitored by measuring luciferase activity. Figure 1. Schematic of the lenti-vector used to generate the ATF6 luciferase reporter lentivirus.