Anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3zeta, eGFP)

The anti-CD19 CAR lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to ready to transduce most mammalian cells, including primary and non-dividing cells. These viruses transduce the ScFv portion of anti-CD19 (clone FMC63) linked to 2nd generation CAR (Chimeric Antigen Receptor), containing CD8 hinge and transmembrane domains, 4-1BB and CD3zeta signaling domains. This construct also includes an IRES- enhanced green fluorescent protein (eGFP) sequence downstream of anti-CD19 CAR cassette to facilitate analysis and sorting of transduced cells (Figure 1). Figure 1. (A) Schematic of the lenti-vector used to generate the anti-CD19 CAR lentivirus. The vector is a SIN vector, and it contains eGFP. (B) Construct diagram showing components of the anti-CD19 CAR. Note: This product transduces the same anti-CD19 CAR construct (CD19 ScFv-CD8-4-1BB-CD3zeta) as other available anti-CD19 CAR Lentiviruses (BPS Bioscience #78600, 78601 and 78602), but differ in key aspects. This product contains an eGFP reporter/selection marker so the transduced cells can be sorted based on eGFP expression. Please see table below. BPS Bioscience Catalog # Self-Inactivation (SIN) Selection Marker 78600 no puromycin 78601 yes no 78602 yes puromycin 78775 yes eGFP