Firefly Luciferase-mCherry Lentivirus (Puromycin)

Firefly Luciferase-mCherry Lentivirus (Hygromycin or Puromycin) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses contain firefly luciferase and mCherry (Luc2-P2A-mCherry) driven by an EF1a promoter, and a hygromycin or puromycin selection marker (Figure 1). Luciferase and mCherry are co-expressed under the EF1A promoter in cells, allowing for flexibility in the detection of transduced cells. Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase-mCherry Lentivirus (Puromycin). This is a SIN vector. Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase-mCherry Lentivirus (Hygromycin). This is a SIN vector.