WM115 Purified Genomic DNA

Genomic DNA was purified from cells using genomic DNA preparaton kit according to manufacturers instruction. DNA was diluted to 200 ng/µL in TE buffer (0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6). Concentration was determined at A260 using nanodrop ND-1000. DNA was prepared from cell line WM115. WM115 is a tumorigenic (VGP) primary melanoma cell line with competence for metastasis. This cell line was established from a metastatic site (right anterior leg) in a 55-year-old female with superficial spreading melanoma. The subject displayed VGP with a Clark level III tumor with thickness of 2.24mm.This cell line features the specific V600D (Val600Asp) mutation at codon 600 in the BRAF gene. This cell line also expresses PTEN loss of function including hemizygous deletion. This cell line was derived from the same patient as the cell lines WM293A, WM165-1 and WM266-4. WM115 cell line originated from the primary tumor, and WM293A, WM165-1 and WM266-4 were from individual lymph-node metastases. WM115 cell line is wild type for N-RAS, c-KIT, and CDK4 genes. WM115 cells produce xenograft tumors when injected into immunocompromised mice.