Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such... read more »
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Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

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Seminal Vesicles, Ram, Frozen
Seminal Vesicles, Ram, Frozen

Item number: S0680.250

Application:, Excellent source for isolation of several eicosanoid metabolizing enzymes. Other applications not tested. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original...
1,094.00€ *
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Spleen Extract, Bovine, Purified
Spleen Extract, Bovine, Purified

Item number: S5550-05A.1

Activities include Ro (SS-A), La (SS-B), Sm, and RNP, Reconstitution: Reconstitute using 1ml of ddH2O. Vortexing will speed dissolution. Applications: Optimal for autoantigen activity in immunodiffusion assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the...
500.00€ *
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STAT3 Control Cell Extracts,
STAT3 Control Cell Extracts,

Item number: S7971-45.10

Stat3 is a key signaling molecule for many cytokines and growth-factor receptors and is required for murine fetal development. Additionally, Stat3 is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. Stat3 is activated by tyrosine phosphorylation at...
462.00€ *
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THP-1 Cell Lysate (Human Monocyte), Lysate
THP-1 Cell Lysate (Human Monocyte), Lysate

Item number: T5019-01.200

Permanent murine cell lines of fast and slow skeletal myocyte derivation. Storage and Stability: For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable...
Application: WB
Species reactivity: human
324.00€ *
Review
THP-1 Nuclear Extract, PMA stimulated (Human Monocyte)
THP-1 Nuclear Extract, PMA stimulated (Human Monocyte)

Item number: T5019-01A.200

Permanent murine cell lines of fast and slow skeletal myocyte derivation. Nuclear extracts have been collected in Lysis Buffer after a 24-hour incubation with PMA (10ng/ml). , Storage and Stability, For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of...
Application: WB
Species reactivity: human
324.00€ *
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L3MBTL1 Positive Control
L3MBTL1 Positive Control

Item number: Cay601033-160

Formulation: A lyophilized powder. Origin: Animal/Bovine.
Application: Kit component
Species reactivity: human
From 34.00€ *
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ENA Extract, Bovine, Rabbit
ENA Extract, Bovine, Rabbit

Item number: 208553.250

500.00€ *
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Epithelial Cell Extract, Human
Epithelial Cell Extract, Human

Item number: 208554.1

Application: WB
318.00€ *
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HeLa Cell Extract
HeLa Cell Extract

Item number: 138011.100

A HeLa cell (also Hela or hela cell) is a cell type in an immortal cell line used in scientific research. It is one of the oldest and most commonly used human cell lines. The line was derived from cervical cancer cells taken from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The...
811.00€ *
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HeLa Whole Cell Lysate Trichostatin A Stimulated
HeLa Whole Cell Lysate Trichostatin A Stimulated

Item number: W09-001-GT2

The cells were grown in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum. Cells were treated with 121ng/mL of Trichostatin A overnight. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0%...
Application: WB
Host: Human
Species reactivity: human
302.00€ *
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HeLa Whole Cell Lysate
HeLa Whole Cell Lysate

Item number: Cay31640-200

HeLa whole cell lysate is intended for use as a control for various biological assays.Source: Human cervix epitheloid carcinoma cell line. Formulation: Lysed in diluted RIPA Buffer (Item No. 10010263) including protease inhibitors.
Application: WB control
205.00€ *
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K562 Whole Cell Lysate
K562 Whole Cell Lysate

Item number: Cay31741-200

K562 whole cell lysate is intended for use as a control for various biological assays.Source: Human myelogenous erythroleukemia cell line. Formulation: Lysed in diluted RIPA Buffer (Cayman Item No: 10010263) including protease inhibitors..
Application: WB control
205.00€ *
Review
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