Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such... read more »
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Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

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HeLa Nuclear Extract
HeLa Nuclear Extract

Item number: H1838.50

Nuclear extract is prepared from human HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins and transcription factors. Applications: Suitable for use as a positive control for antibodies. Recommended Usage: Add an equal volume of Laemmli reducing sample buffer to...
Application: WB
568.00€ *
Review
Helicobacter pylori, Lysate
Helicobacter pylori, Lysate

Item number: H1840-04.1

Helicobacter pylori is a spiral-shaped, Gram-negative rod approximately 0.5x3.0um in size. The catalase-positive organism has 4-6 sheathed flagella attached to one pole which allow for motility. Helicobacter pylori lives in the stomach and duodenum (section of intestine just below stomach). It has a unique way of...
Application: ELISA
978.00€ *
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Helicobacter pylori, Purified
Helicobacter pylori, Purified

Item number: H1840.1

Helicobacter pylori is a spiral-shaped, Gram-negative rod approximately 0.5x3.0um in size. The catalase-positive organism has 4-6 sheathed flagella attached to one pole which allow for motility. Helicobacter pylori lives in the stomach and duodenum (section of intestine just below stomach). It has a unique way of...
Application: ELISA
780.00€ *
Review
Inducible Nitric Oxide Synthase, Western Blot Standard (iNOS, iNOS 2, mNOS)
Inducible Nitric Oxide Synthase, Western Blot Standard...

Item number: I0800-08R.50

Recommended Dilution:, Western Blot: 5-10ul per lane., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the...
Application: WB
390.00€ *
Review
Inducible Nitric Oxide Synthase, Western Blot Standard (iNOS, iNOS 2, mNOS)
Inducible Nitric Oxide Synthase, Western Blot Standard...

Item number: I0800-08S.50

Applications: , Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: Use 5-10ul per lane., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable...
Application: WB
390.00€ *
Review
Jurkat Cell, Lysate
Jurkat Cell, Lysate

Item number: J9000.100

Cellular Protein Preparation: , Cells were lysed in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM sodium chloride, 1mM EDTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin, 1mM sodium orthovanadate, 1mM sodium fluoride). Diluted with non-reducing sample buffer...
Application: WB
591.00€ *
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MAP Kinase Kinase 3,6 (MKK3,6) Control Cell Extracts,
MAP Kinase Kinase 3,6 (MKK3,6) Control Cell Extracts,

Item number: M2363-15B.10

MAP Kinase Kinase 3,6 (MKK3,6) Control Cell Extracts, Form: Supplied as a liquid in SDS Sample Buffer: 62.5mM Tris-HCl, pH 6.8 at 25°C, 2% SDS, 10% glycerol, 50mM DTT, 0.01% bromophenol blue or phenol red. Kit Components: (Sufficient for 10 blots) M2363-15B1: MAP Kinase Kinase 3,6 (MKK3,6) Nonphosphorylated Control...
Application: WB
537.00€ *
Review
Mumps, Highly Purified
Mumps, Highly Purified

Item number: M9215.1

Mumps virus is a member of the Paramyxoviridae family. There is only one serotype. The virion is pleomorphic with a roughly spherical shape and a diameter of 85-300um. The nucleocapsid is composed of a continuous linear molecule of single-stranded RNA that is tightly coiled in a helical formation and a capsid...
Application: ELISA
629.00€ *
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Parainfluenza Virus Type 3, Strain C243, Lysate
Parainfluenza Virus Type 3, Strain C243, Lysate

Item number: P3107-15A.1

Source: Vero cells , Inactivation: Inactivated using gamma radiation., Purity: Optimally infected monolayers are harvested, disrupted by sonication and subjected to low speed centrifugation. The clarified cell lysate is pooled with supernatant from the infected culture and concentrated using crossflow...
Application: Antigen preparation
788.00€ *
Review
Phosphotyrosine Control, EGF-Stimulated A431 Cell, Lysate
Phosphotyrosine Control, EGF-Stimulated A431 Cell, Lysate

Item number: P4078-10.1

Applications: , Positive control for anti-phosphotyrosine antibodies. Suitable for use in Western Blot, Immunoprecipitation. Recommended Dilution: Western Blot: Detects the phosphorylated EGF receptor (170kD) and other tyrosine phosphorylated proteins in 20ug of the preparation. Add an equal volume of Laemmli...
Application: IP, WB
894.00€ *
Review
Rubeola, Strain Edmonston (Measles)
Rubeola, Strain Edmonston (Measles)

Item number: R9750-11F.1

Measles (Rubeola) Virus is a community-related acute disease commonly known as red measles or seven-day measles. Measles is one of the infectious diseases of childhood causing a skin rash. Serious complications, such as pneumonia, croup or encephalitis, can occur. It is highly contagious and associated with high...
Application: ELISA
606.00€ *
Review
Seminal Vesicles, Ram, Frozen
Seminal Vesicles, Ram, Frozen

Item number: S0680.250

Application:, Excellent source for isolation of several eicosanoid metabolizing enzymes. Other applications not tested. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original...
1,094.00€ *
Review
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