WM3758 Viable Cells

Cells are sterile, validated by short tandem repeat profiling, and are tested as negative for mycoplasma. It is recommended that cell lines are tested for mycoplasma contamination and short tandem repeat (STR) profiling every 10 passages or each time a frozen seed stock is made. See cell culture protocol for additional details. WM3758 is a metastatic human melanoma cell line. This cell line contains a Q61L mutation at position 61 in the N-RAS gene. The Q61L mutation results in an amino acid substitution at position 61 in NRAS, from a glutamine (Q) to a leucine (L). This cell line expresses wild type BRAF, c-KIT, and CDK4 genes. WM3758 cells match with WM3682 and WM3702. These cells produce xenograft tumors when injected into immunocompromised mice.