WM3702 Viable Cells

Cells are sterile, validated by short tandem repeat profiling, and are tested as negative for mycoplasma. It is recommended that cell lines are tested for mycoplasma contamination and short tandem repeat (STR) profiling every 10 passages or each time a frozen seed stock is made. See cell culture protocol for additional details. WM3702 is a metastatic human melanoma cell line that displays pigmented, thin elongated morphology in culture. This cell line was established from a right upper flank of back in a 58-year-old male with necrotic fungating melanoma. The subject displayed metastatic melanoma forming multiple subcutaneous nodules with overlying skin ulcerations. This cell line contains a Q61L mutation at position 61 in the N-RAS gene. The Q61L mutation results in an amino acid substitution at position 61 in NRAS, from a glutamine (Q) to a leucine (L). WM3702 cells match with WM3702 and WM3682. These cells produce xenograft tumors when injected into immunocompromised mice.