WM3456 Non-Viable Cell Pellet

Cells were grown sub-confluent to approximately (2x10^6) cells in Tumor specialized media and then trypsinized. Cells were harvested and cell pellets were flash frozen and stored at -80°C. Non-viable cell pellet was generated from cell line WM3456. WM3456 is a human metastatic melanoma cell line that displays a thin elongated, fibroblastic morphology. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation.  WM3456 cells produce xenograft tumors when injected into immunocompromised mice.