CRISPRa (SAM) HeLa Cell Line

CRISPRa (SAM) HeLa Cell Line
Item number Size Datasheet Manual SDS Delivery time Quantity Price
BPS-78193 2 vials - -

3 - 15 business days*

3,048.00€
 
This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM)... more
Product information "CRISPRa (SAM) HeLa Cell Line"
This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR associated protein 9), lacking any endonuclease activity, fused to transcriptional activator VP64. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express P65 (Transcription Factor p65, or Nuclear Factor NF-kappaB p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag, which is maintained with Hygromycin resistance. When these cells are transfected with an MS2- tagged sgRNA targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the genomic DNA and begin transcription, inducing expression of the desired gene.
Keywords: SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1,
Supplier: BPS Bioscience
Supplier-Nr: 78193

Properties

Application: CRISPR studies
Reactivity: Streptococcus Pyogenes Serotype M1

Database Information

KEGG ID : K09952 | Find alternatives
UniProt ID : Q99ZW2 | Find alternatives

Handling & Safety

Storage: Liquid nitrogen
Shipping: -80°C (International: °C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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