TNFSF4/OX40L BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human TNFSF4/OX40L in cell culture supernates, serum and plasma (heparin, EDTA). OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, the mouse Tnfsf4 gene is mapped to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene. OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans. , Assay Range:, 156pg/ml-10,000pg/ml, Sensitivity: < 10 pg/ml , Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P23510, Entrez Gene ID: 7292, Kit Components: 96-well plate pre-coated with anti-TNFSF4/OX40L, Human, Lyophilized recombinant TNFSF4/OX40L, Human standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.