S100A9, Human, BioAssay(TM) DuoSet ELISA Development Kit (Calgranulin B, MRP-14)

Human S100A9 (also MRP-14, Calgranulin-B, and p14) is a 14kD member of the S100 family of EF-hand calcium-binding proteins. It is 114 amino acids (aa) in length and contains short sequential modules. There is an N-terminal helical region, followed by a calcium-binding EF-hand domain, two more helical regions, a second EF-hand domain, and three additional helical regions. S100A9 will noncovalently heterodimerize with S100A8. In the presence of calcium, this heterodimer will form a heterotetramer. S100A9 is expressed in granulocytes, monocytes, and macrophages during acute and chronic inflammation. Human S100A9 shares 62% and 57% aa identity with rat and mouse S100A9, respectively. Sample Type: Cell culture supernates, Intended Use: This BioAssay(TM) DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human S100 Calcium Binding Protein A9 (S100A9). DuoSets are designed for the analysis of cell culture supernates. Range: ~31.2-2000pg/ml, Specificity: Recognizes natural and recombinant human S100 Calcium Binding Protein A9 (S100A9). Kit Components: S100A9 capture antibody, 1x vial, Biotinylated mouse anti-human S100A9 detection antibody, 1x vial, Recombinant human S100A9 standard, 1x 3vials, Streptavidin-HRP, 1x vial, Storage and Stability: Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.