Rat HMGB-1 (HighMobility Group Protein B1) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve.. Detection Range: 15.63-1000 pg/mL. Sensitivity: 6.7 pg/mL. Assay Type: Sandwich. Standard: 1000 pg/mL. Sample Type: serum, plasma, tissue homogenates and other biological fluids. Assay duration: 3.5h