Prostaglandin I Metabolite ELISA Kit

Prostaglandin I (Prostaglandin I2, PGI2) is a potent vasodilator and inhibitor of platelet aggregation that is formed from arachidonic acid primarily in the vascular endothelium and renal cortex by sequential activities of cyclooxygenase (COX) and PGI2 synthase. It is non-enzymatically hydrated to 6-keto PGF1alpha (t1/2 = 2-3 minutes), and then quickly converted to numerous downstream metabolites, collectively known as prostaglandin I metabolites or PGIM, (t1/2 = 30 minutes). Although 6-keto PGF1alpha is commonly measured in plasma and urine as an estimate of PGI2 synthesis, it should be noted that there may be more than one source of PGI2 in these samples. For instance, urinary concentrations of 6-keto PGF1alpha are confounded by the fact that only a portion originates from plasma and the remainder is produced by the kidney. These problems are circumvented by measuring downstream PGIM rather than 6-keto PGF1alpha as an indicator of systemic PGI2 production. Cayman's PGIM ELISA Kit is a competitive assay that can be used for quantification of PGIM in urine and other sample matrices. The assay has a range from 39-5,000 pg/ml and a sensitivity (80% B/B0) of approximately 120 pg/ml.Synonyms: PGIM EIA Kit. Assay Range: 39-5,000 pg/ml. Sensitivity: 80% B/B0: 120 pg/ml. Mid-point: 50% B/B0: 500 pg/ml. Cross Reactivity: (2,3-dinor-6-keto Prostaglandin F1alpha) 100%, (20-carboxy-2,3-dinor-6-keto Prostaglandin F1alpha) 234%, (2,3-dinor-11beta-Prostaglandin F2alpha) 0.3%, (tetranor-PGFM) 0.07%, (8-Isoprostane) <0.01%, (Prostaglandin B1) <0.01%, (Prostaglandin B2) <0.01%, (Prostaglandin E1) <0.01%, (Prostaglandin E2) <0.01%, (Prostaglandin F1alpha) <0.01%, (13,14-dihydro-Prostaglandin F1alpha) <0.01%, (6,15-diketo-13,14-dihydro-Prostaglandin F1alpha) <0.01%, (6-keto Prostaglandin F1alpha) <0.01%, (Prostaglandin F2alpha) <0.01%, (5-keto Prostaglandin F2alpha) <0.01%, (Thromboxane B2) <0.01%. Origin: Animal/Bovine, Animal/Eel, Animal/Mouse.