Phospho-p38 alpha (T180/Y182) BioAssay(TM) DuoSet IC ELISA Kit (MAPK14, SAPK2a)

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses. All four p38 family members, alpha, beta, gamma, and delta, are phosphorylated by MKK3 and/or MKK6 at dual Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr. Once activated, p38 phosphorylates a number of targets, including the nuclear transcription factors ATF2 and Max.The most frequently analyzed family member, p38 alpha, also known as SAPK2a and MAPK14, was initially purified as a kinase critical to the signaling cascade linking IL-1 to MAPKAPK-2 and the small heat shock protein HSP27. Ubiquitously expressed, p38 alpha is dually phosphorylated by MKK3 and MKK6 at Thr180 and Tyr182. Once activated, p38 alpha phosphorylates a number of targets, including the cytoplasmic kinases MNK 4 and PRAK5 and the nuclear transcription factors ATF2 1 and STAT1. Several promising compounds that inhibit p38 alpha are being investigated as potential therapies for arthritic and inflammatory diseases. Sample Type: Cell lysates, Range: ~62.5-4000pg/ml, Specificity: The human/mouse/rat Phospho-p38a ELISA kit specifically recognizes phosphorylated p38a. Specificity was demonstrated by Western blot analysis of the protein bound by the capture antibody supplied in the kit. Test Principle: The BioAssay(TM) ELISA kit contains the basic components required for the development of sandwich ELISAs to measure p38a phosphorylated at T180/Y182 in cell lysates. An immobilized capture antibody specific for p38a binds both phosphorylated and unphosphorylated p38a. After washing away unbound material, a biotinylated detection antibody specific for p38a dually phosphorylated at T180 and Y182 is used to detect captured protein, utilizing a standard Streptavidin-HRP format. Kit Components: Phospho-p38a Capture Antibody, 1x vial, Phospho-p38a Detection Antibody, 1x vial, Phospho-p38a Standard, 3x vials, Streptavidin-HRP, 1x vial, Storage and Stability: Store powder at 4°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.