Cookie preferences
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the comfort when using this website, are used for direct advertising or to facilitate interaction with other websites and social networks, are only set with your consent.
Configuration
Technically required
These cookies are necessary for the basic functions of the shop.
"Allow all cookies" cookie
"Decline all cookies" cookie
CSRF token
Cookie preferences
Currency change
Customer-specific caching
FACT-Finder tracking
Individual prices
Selected shop
Session
Comfort functions
These cookies are used to make the shopping experience even more appealing, for example for the recognition of the visitor.
Note
Show the facebook fanpage in the right blod sidebar
Statistics & Tracking
Affiliate program
Conversion and usertracking via Google Tag Manager
Track device being used
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
145759.1 | 1 kit | - | - |
3 - 19 business days* |
802.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases... more
Product information "Phospho-p38 alpha (T180/Y182) BioAssay(TM) DuoSet IC ELISA Kit (MAPK14, SAPK2a)"
The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses. All four p38 family members, alpha, beta, gamma, and delta, are phosphorylated by MKK3 and/or MKK6 at dual Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr. Once activated, p38 phosphorylates a number of targets, including the nuclear transcription factors ATF2 and Max.The most frequently analyzed family member, p38 alpha, also known as SAPK2a and MAPK14, was initially purified as a kinase critical to the signaling cascade linking IL-1 to MAPKAPK-2 and the small heat shock protein HSP27. Ubiquitously expressed, p38 alpha is dually phosphorylated by MKK3 and MKK6 at Thr180 and Tyr182. Once activated, p38 alpha phosphorylates a number of targets, including the cytoplasmic kinases MNK 4 and PRAK5 and the nuclear transcription factors ATF2 1 and STAT1. Several promising compounds that inhibit p38 alpha are being investigated as potential therapies for arthritic and inflammatory diseases. Sample Type: Cell lysates, Range: ~62.5-4000pg/ml, Specificity: The human/mouse/rat Phospho-p38a ELISA kit specifically recognizes phosphorylated p38a. Specificity was demonstrated by Western blot analysis of the protein bound by the capture antibody supplied in the kit. Test Principle: The BioAssay(TM) ELISA kit contains the basic components required for the development of sandwich ELISAs to measure p38a phosphorylated at T180/Y182 in cell lysates. An immobilized capture antibody specific for p38a binds both phosphorylated and unphosphorylated p38a. After washing away unbound material, a biotinylated detection antibody specific for p38a dually phosphorylated at T180 and Y182 is used to detect captured protein, utilizing a standard Streptavidin-HRP format. Kit Components: Phospho-p38a Capture Antibody, 1x vial, Phospho-p38a Detection Antibody, 1x vial, Phospho-p38a Standard, 3x vials, Streptavidin-HRP, 1x vial, Storage and Stability: Store powder at 4°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: | United States Biological |
Supplier-Nr: | 145759 |
Properties
Database Information
Handling & Safety
Storage: | +4°C |
Shipping: | +4°C (International: °C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
more
You will get a certificate here
Viewed