Mouse NFKB-p65 (Nuclear Factor Kappa B p65) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFKB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFKB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFKB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFKB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.. Detection Range: 31.25-2000 pg/mL. Sensitivity: 11.2 pg/mL. Assay Type: Sandwich. Standard: 2000 pg/mL. Sample Type: Tissue homogenates, cell lysates and other biological fluids. Assay duration: 3.5h