MIS/AMH, Human, BioAssay(TM) DuoSet ELISA Development Kit

Müllerian inhibiting substance (MIS), also named anti-Müllerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to Sertoli cells of fetal and postnatal testis, and to granulosa cells of postnatal ovary. MIS is synthesized and secreted as a disulfide-linked homodimeric propeptide. Proteolytic cleavage is required to separate the N-terminal proregion from the C-terminal bioactive peptide. Both fragments remain associated in a non-covalent complex. The C-terminal sequence forms a cysteine knot via seven canonical cysteine residues found in TGF-beta superfamily members. Sample Type: Cell culture supernates. Intended Use: This BioAssay(TM) DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Mullerian-inhibiting Substance (MIS). DuoSets are designed for the analysis of cell culture supernates. Range: 93.8-6000pg/ml, Specificity: Natural and recombinant human Mullerian-inhibiting Substance (MIS). Kit Components: Mouse anti-human MIS capture antibody, 1x vial, Biotinylated mouse anti-human MIS detection antibody, 1x vial, Recombinant human MIS standard, 1x 3vials, Streptavidin-HRP, 1x vial, Storage and Stability: Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.