Kallistatin/Serpina4 BioAssay(TM) ELISA Kit, Human

Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth. Kallistatin/Serpina4 BioAssay(TM) ELISA Kit is a sandwich ELISA for quantitative detection of human Kallistatin/Serpina4 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). , Range:, 156pg/ml-10,000pg/ml, Sensitivity: <10 pg/ml , Specificity: Natural and recombinant human Kallistatin/Serpina4, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Kit Components: 143938A: Microtiter Strips, 1x96 wells, 143938B: Kallistatin/Serpina4, Standard, 2x10ng, 143938C: Anti-human Kallistatin/Serpina4 (Biotin), 1x130ul (1:100 dilution), USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample Diluent Buffer, 1x30ml, 143698: Antibody Diluent Buffer, 1x12ml, 143699: ABC Diluent Buffer, 1x12ml, 143700: TMB Color Developing Agent, 1x10ml, 143701: TMB Stop Solution, 2N H2SO4, 1x10ml, Storage and Stability: Store *143938B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. , 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS or PBS., 3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS or PBS., 4. Add TMB color developing agent and incubate the plate at 37°C in dark for 15-20 minutes., 5. Add TMB stop solution and read.