Insulin Like Growth Factor Binding Protein 5 (IGFBP5), BioAssay(TM) ELISA Kit (Rat)

Insulin Like Growth Factor Binding Protein 5 (IGFBP5), BioAssay(TM) ELISA Kit (Rat)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
352943.96 96 tests - -

3 - 19 business days*

947.00€
 
Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5... more
Product information "Insulin Like Growth Factor Binding Protein 5 (IGFBP5), BioAssay(TM) ELISA Kit (Rat)"
Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA), Intended Use: For quantitative detection of rat IGFBP5 in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <10pg/ml, Range: 156-10,000pg/ml, Specificity: Natural and recombinant rat IGFBP5, Test Principle: This rat IGFBP5 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP5 has been precoated onto 96-well plates. Standards(NSO, L20-E271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IGFBP5 amount of sample captured in plate. Kit Components: 96-well plate precoated with rat IGFBP5 antibody, 1, ST0000-20, Lyophilized recombinant rat IGFBP5 standard, 20ng/tube×2, Biotinylated rat IGFBP5 antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: IBP-5, Igfbp5, Igfbp-5, IGFBP-5, IGF-binding protein 5, Insulin-like growth factor-binding protein 5
Supplier: United States Biological
Supplier-Nr: 352943

Properties

Application: ELISA
Species reactivity: rat
Format: Solid Phase

Handling & Safety

Storage: vT
Shipping: +4°C (International: °C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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