IL-10 (Interleukin 10) BioAssay(TM) ELISA Kit (Equine)

Sample Type:, Serum, plasma, tissue homogenates and other biological fluids. Intended Use: Sandwich ELISA, Double Antibody, Sensitivity: <9.375pg/ml, Range: 15.625-1000pg/ml, Specificity: Equine, Test Principle: This BioAssay(TM) kit was based on sandwich enzyme-linked immune-sorbent assay technology. IL-10 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-10 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-10 can be calculated. Kit Components: Micro ELISA Plate (Dismountable), 8x12, Lyophilized Standard, 2xvial, Sample/Standard dilution buffer, 20ml, Biotin-detection antibody (Concentrated), 120ul, Antibody dilution buffer, 10ml, HRP-Streptavidin Conjugate (SABC), 120ul, SABC dilution buffer, 10ml, TMB substrate, 10ml, Stop solution, 10ml, Wash buffer (25X), 30ml, Plate Sealer, 5xpieces, Product Description, 1xcopy, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.