Hepcidin (Hepc) High Sensitivity BioAssay(TM) ELISA Kit (Rat)

Sample Type:, Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids, Intended Use: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of hepcidin in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Sensitivity: 87.3pg/ml, Range: 246.9-20,000pg/ml, Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Hepcidin (Hepc). No significant cross-reactivity or interference between High Sensitive Hepcidin (Hepc) and analogues was observed. Precision: Intra-Assay: CV<10% , Inter-Assay: CV<12%, Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to hepcidin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled hepcidin and unlabeled hepcidin (Standards or samples) with the pre-coated antibody specific to hepcidin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of hepcidin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of hepcidin in the sample. Kit Components: 96-well strip plate, 1x Plate , Standard, 2x vial, Detection Reagent A, 1×40ul, Detection Reagent B, 1×120ul, TMB Substrate, 1×9ml, Wash Buffer (30X), 1×20ml, Standard Diluent, 1×20ml, Assay Diluent A, 1×12ml, Assay Diluent B, 1×12ml, Stop Solution, 1x6ml, Storage and Stability: Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.