CYR61/CCN1, BioAssay(TM) ELISA Kit (Human)

Cysteine-rich angiogenic inducer 61(CYR61) or CCN family member 1(CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers. Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and human milk, Intended Use: For quantitative detection of human CYR61 in cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: <10pg/ml, Range: 31.2-2000pg/ml, Specificity: Natural and recombinant human CYR61, Test Principle: This human CYR61 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CYR61 has been precoated onto 96-well plates. Standards(CHO) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CYR61 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CYR61 amount of sample captured in plate. Kit Components: 96-well plate precoated with human CYR61 antibody, 1, Lyophilized recombinant human CYR61 standard, 10ng/tube×2, Biotinylated human CYR61 antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.