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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
352784.96 | 96 tests | - | - |
3 - 19 business days* |
947.00€
|
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You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2... more
Product information "Chemerin/RARRES2, BioAssay(TM) ELISA Kit (Human)"
Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1(also known as ChemR23). It is a 14kD protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What's more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA), Intended Use: For quantitative detection of human Chemerin in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <20pg/ml, Range: 0.78-50ng/ml, Specificity: Natural and recombinant human Chemerin, Test Principle: This human Chemerin BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Chemerin has been precoated onto 96-well plates. Standards(E.coli, V17-S163) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chemerin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Chemerin amount of sample captured in plate. Kit Components: 96-well plate precoated with human Chemerin antibody, 1, Lyophilized recombinant human Chemerin standard, 50ng/tube×2, Biotinylated human Chemerin antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: | TIG2, RARRES2, Chemerin, RAR-responsive protein TIG2, Tazarotene-induced gene 2 protein, Retinoic acid receptor responder protein 2 |
Supplier: | United States Biological |
Supplier-Nr: | 352784 |
Properties
Application: | ELISA |
Host: | Human |
Species reactivity: | human |
Format: | Solid Phase |
Database Information
KEGG ID : | K10044 | Matching products |
UniProt ID : | Q99969 | Matching products |
Gene ID | GeneID 5919 | Matching products |
Handling & Safety
Storage: | vT |
Shipping: | +4°C (International: °C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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