Annexin A1 (ANXA1) BioAssay(TM) ELISA Kit (Human)

Annexin A1 (ANXA1) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ANXA1 in human serum, plasma, tissue homogenates and other biological fluids. Detection Range: 0.312-20ng/ml, Sensitivity: 0.115ng/ml, Intra-Assay CV: <10% , Inter-Assay CV: <12%, Kit Components: *023428A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use, *023428B: Standard, 2x1vial, 023428C: Standard Diluent, 1x10ml or 1x20ml, *023428D: Detection Reagent A (green), 1x60ul or 1x120ul, *023428E: Detection Reagent B (red), 1x60ul or 1x120ul, 023428F: Assay Diluent A, 1x6ml or 1x12ml, 023428G: Assay Diluent B, 1x6ml or 1x12ml, 023428H: TMB Substrate, 1x4.5ml or 1x9ml, 023428J: Positive Control 2.11ng/ml, 1x1vial (Lyophilized), 023428K: Stop Solution, 1x3ml or 1x6ml, 023428L: Wash Buffer, 30X, 1x10ml or 1x20ml, Precaution: The Stop Solution (023428K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *023428A, *023428B, *023428D, *023428E and *023428J at -20°C. After reconstitution of *023428-SP-J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once opened, it is highly recommended to use remaining reagents within 1 month. , Assay Procedure Summary:, 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C., 3. Add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.