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Product information "13,14-dihydro-15-keto Prostaglandin F2alpha ELISA Kit"
Prostaglandin F2alpha (PGF2alpha) is one of the five primary prostaglandins derived enzymatically directly from the endoperoxide PGH2. PGF2alpha was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF2alpha is a potent luteolytic agent and is used to induce ovulation in domestic livestock. It is also a potent uterine stimulant, and is part of the cascade of myometrial stimulants which induce and sustain labor. PGF2alpha is rapidly metabolized to 13,14-dihydro-15-keto PGF2alpha in vivo, by the enzymes 15-hydroxy prostaglandin dehydrogenase and Delta13-reductucase. Measurement of 13,14-dihydro-15-keto PGF2alpha in plasma can be used as a marker of the in vivo production of PGF2alpha. The Cayman Chemical 13,14-dihydro-15-keto PGF2alpha Enzyme Immunoassay is a competitive assay that provides measurements of 13,14-dihydro-15-keto PGF2alpha within the range of 2.3-5,000 pg/ml, typically with a detection limit (80% B/B0) of approximately 15 pg/ml. Although the tracer and antiserum for this assay have been sold separately for many years, the reagents are now combined with an accurate standard and packaged together in a convenient assay kit. The assay has been validated for the measurement of 13,14-dihydro-15-keto PGF2alpha in plasma, a common matrix for measurement of this PGF2alpha metabolite.Synonyms: 13,14-dihydro-15-keto PGF2alpha EIA Kit, PGFM EIA Kit. Assay Range: 2.3-5,000 pg/ml. Sensitivity: 80% B/B0: 15 pg/ml. Mid-point: 50% B/B0: 120-180 pg/ml. Cross Reactivity: (13,14-dihydro-15-keto Prostaglandin F2alpha) 100%, (13,14-dihydro-15-keto Prostaglandin E2) 2.7%, (15-keto Prostaglandin F2alpha) 1.8%, (Prostaglandin D2) <0.01%, (Prostaglandin E2) <0.01%, (Prostaglandin F1alpha) <0.01%, (2,3-dinor-6-keto Prostaglandin F1alpha) <0.01%, (6-keto Prostaglandin F1alpha) <0.01%, (Prostaglandin F2alpha) <0.01%, (8-iso Prostaglandin F2alpha) <0.01%, (8-iso-13,14-dihydro-15-keto Prostaglandin F2alpha) <0.01%, (8-iso-15-keto Prostaglandin F2alpha) <0.01%, (Prostaglandin F2beta) <0.01%, (8-iso Prostaglandin F3alpha) <0.01%, (Thromboxane B2) <0.01%. Origin: Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit.
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the usability of this website, serve for direct advertising or simplify interaction with other websites and social networks, will only be used with your consent.
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