Uridine Diphosphate Glucose Pyrophosphorylase (UGP) Activity Assay Kit

Detection principle: Uridine diphosphate glucose pyrophosphorylase (UGP) catalyzes the synthesis of 1-phosphoglucose in the sample into UDP-glucose, and UDP-glucose is catalyzed by the enzyme to produce a color producing substance, which has a maximum absorption peak at about 450 nm. The enzyme activity can be calculated by the increase of OD value at 450 nm. Performance characteristics: Sample type: animal tissue, plant tissue. Sensitivity: 0.5 U/L. Detection range: 0.5-70.0 U/L. Detection method: Colorimetric method. Assay type: Enzyme Activity. Assay time: 35 min. Precision: Average inter-assay CV: 2.3-3.9%, Average intra-assay CV: 1.0-3.2%. Storage: -20?. Valid period: 12 months. Protein function: UTP--glucose-1-phosphate uridylyltransferase catalyzing the conversion of glucose-1-phosphate into UDP-glucose, a crucial precursor for the production of glycogen. [The UniProt Consortium]