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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
BPS-78851 | 384 rxns | - | - |
3 - 15 business days* |
2,657.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The TNF receptor associated factor 6 (TRAF6) TR-FRET Assay Kit is a sensitive TR-FRET Assay Kit,... more
Product information "TRAF6 TR-FRET Assay Kit"
The TNF receptor associated factor 6 (TRAF6) TR-FRET Assay Kit is a sensitive TR-FRET Assay Kit, designed to measure TRAF6 (amino acids 51-159) auto-ubiquitination activity in a homogeneous 384 reaction format. This assay measures mono- or poly-ubiquitination. As a homogeneous assay, it requires no time-consuming washing steps and is especially suitable for high throughput screening (HTS) applications as well as real-time analyses. Read out requires a fluorescent plate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET). The assay takes advantage of biotinylated ubiquitin, which is linked to TRAF6 during auto-ubiquitination. The streptavidin-conjugated acceptor binds to ubiquitin-biotin while a Terbium-conjugated anti-GST antibody donor binds to GST-tagged TRAF6, allowing TR-FRET pairing. The resulting TR-FRET signal is directly proportional to TRAF6 mono or poly-ubiquitination.
Keywords: | TRAF6, RNF85, EC=2.3.2.27, RING finger protein 85, Interleukin-1 signal transducer, TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, RING-type E3 ubiquitin transferase TRAF6, |
Supplier: | BPS Bioscience |
Supplier-Nr: | 78851 |
Properties
Application: | Ubiquitination studies, auto-ubiquitination activity assays |
Species reactivity: | human |
Format: | Homog. |
Database Information
KEGG ID : | K03175 | Matching products |
UniProt ID : | Q9Y4K3 | Matching products |
Gene ID | GeneID 7189 | Matching products |
Handling & Safety
Storage: | -80°C |
Shipping: | -80°C (International: °C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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