TAF1L (BD2) Inhibitor Screening Assay Kit

The TAF1L (BD2) Inhibitor Screening Assay Kit is designed to measure the inhibition of TAF1L binding to its substrate. This assay kit comes in a convenient AlphaLISA format with enough biotinylated histone peptide substrate, assay buffer, detection buffer, and purified GST-tagged TAF1L bromodomain to perform a total of 384 enzyme reactions. The key to the assay kit is specific binding of the TAF1L bromodomain 2 (a.a. 1517-1649) to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing TAF1L and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.