Glutathione Peroxidase Assay Kit

Glutathione Peroxidase Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay703102-96 96 wells -

1 - 10 business days

357.00€
Cay703102-480 480 wells -

1 - 10 business days

613.00€
 
Glutathione peroxidase (GPx) catalyzes the reduction of hydroperoxides, including hydrogen... more
Product information "Glutathione Peroxidase Assay Kit"
Glutathione peroxidase (GPx) catalyzes the reduction of hydroperoxides, including hydrogen peroxides, by reduced glutathione and functions to protect the cell from oxidative damage. With the exception of phospholipid-hydroperoxide GPx, a monomer, all of the GPx enzymes are tetramers of four identical subunits. Each subunit contains a selenocysteine in the active site which participates directly in the two-electron reduction of the peroxide substrate. The enzyme uses glutathione as the ultimate electron donor to regenerate the reduced form of the selenocysteine. The Cayman Chemical Glutathione Peroxidase Assay Kit measures GPx activity indirectly by a coupled reaction with glutathione reductase (GR). Oxidized glutathione (GSSG), produced upon reduction of an organic hydroperoxide by GPx, is recycled to its reduced state by GR and NADPH. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the A340 is directly proportional to the GPx activity in the sample. The Cayman GPx Assay Kit can be used to measure all of the glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.
Keywords: EC=1.11.1.9, GPX
Supplier-Nr: 703102-96

Properties

Application: GTx activity detection
Format: Homog.

Database Information

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Signal Word: Warning
GHS Hazard Pictograms:
H Phrases: H319, H335
P Phrases: P261, P264, P271, P280, P312, P304+P340, P305+P351+P338, P337+P313, P405, P403+P233, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
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