Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit

Detection principle: Free fatty acids (NEFA/FFA) generate acyl-CoA under the action of acyl synthase, then acyl-CoA generates hydrogen peroxide under the oxidation of acyl oxidase. Hydrogen peroxide reacts in the presence of enzymes and chromogens, producing a red substance with maximum absorption at 546 nm. The amount of this red product is proportional to the content of free fatty acids. Performance characteristics: Sample type: serum, plasma, animal tissue, cell. Sensitivity: 0.07 mmol/L. Detection range: 0.07-2 mmol/L. Detection method: Colorimetric method. Assay type: Quantitative Assay. time: 30 min Precision: Average inter-assay CV: 2.5-6.1%. Average intra-assay CV: 1.5-4.5%. Other reagents required: Normal saline (0.9% NaCl), Double distilled water. Storage: Reagent 2 -20?, others 2-8?. Valid period: 12 months.