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| Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
|---|---|---|---|---|---|---|---|
| BPS-82219 | 384 rxns | - | - |
3 - 15 business days* |
1,202.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The CUL1 Intrachain TR-FRET Assay Kit is a homogeneous, sensitive TR-FRET (Time-Resolved... more
Product information "CUL1 Intrachain TR-FRET Assay Kit"
The CUL1 Intrachain TR-FRET Assay Kit is a homogeneous, sensitive TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) assay kit designed to measure CUL1 (cullin 1) auto-ubiquitination. It utilizes a Europium-labeled ubiquitin (Ub) donor as well as Cy5-labeled Ub acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on CUL1, this assay measures poly-ubiquitination. The kit comes in a convenient 384-well format and contains enough purified CUL1/SKP1/SKP2/RBX1 Complex, purified UBE1 (ubiquitin-like modifier activating enzyme 1), UbcH5b (Ubiquitin-conjugating enzyme E2 D2), Ubi-Mix(TM), ATP, and assay buffer for 400 reactions. Figure 1. CUL1 Intrachain TR-FRET Assay Kit schematic.E1 and E2 enzymes, Europium cryptate-labeled Ubiquitin (TR-FRET donor) and Cy5-labeled Ubiquitin (TR-FRET acceptor) are incubated with CUL1 complex. The donor and acceptor are incorporated into poly-ubiquitin chains formed on CUL1, allowing energy transfer to occur. The TR-FRET signal is proportional to CUL1 activity
| Keywords: | Cullin-1, CUL1, CUL-1, |
| Supplier: | BPS Bioscience |
| Supplier-Nr: | 82219 |
Properties
| Application: | Inhibitor screening, drug discovery, HTS, IC determination, real-time kinetic studies |
| Format: | Homog. |
Database Information
| KEGG ID : | K03347 | Matching products |
Handling & Safety
| Storage: | -80°C |
| Shipping: | -80°C (International: -80°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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